Spectrofluorimeter

The spectrofluorimeter available at the University of Milano‑Bicocca unit is a Varian Eclipse system designed for high‑sensitivity fluorescence measurements in liquid solutions. It enables the acquisition of excitation and emission spectra of a wide range of fluorophores, supporting routine analyses as well as advanced studies in photophysics, supramolecular chemistry, and bioanalytical applications. The instrument operates with a xenon flash lamp that provides intense, short‑duration excitation pulses, ensuring high signal‑to‑noise ratios and minimizing photobleaching of sensitive samples. Its optical configuration allows rapid scanning across the UV–visible range, enabling kinetic measurements and time‑dependent fluorescence studies. The system supports multiple acquisition modes, including fluorescence, phosphorescence, chemiluminescence, and bioluminescence, offering flexibility for diverse research needs. Temperature‑controlled cuvette holders allow measurements under variable thermal conditions, facilitating studies of thermally activated processes, molecular stability, and conformational changes. The spectrofluorimeter is equipped with software for spectral correction, baseline subtraction, and quantitative analysis, ensuring accurate and reproducible data processing. Accessories such as fiber‑optic probes and solid‑sample holders extend its applicability to heterogeneous materials and in‑situ measurements. Overall, the Varian Eclipse fluorimeter provides a robust and versatile platform for researchers and industrial users requiring precise characterization of fluorescence properties in solution and complex matrices.