DNA Sequencing NGS

General Information

Technique

Materials Processing

Key Instrumentation

Next Generation Sequencing

The Illumina NextSeq 550 is a versatile, high‑performance NGS platform designed to support a broad spectrum of genomic applications for research laboratories and scientific companies. Combining sequencing‑by‑synthesis chemistry with tunable throughput, it enables efficient processing of targeted panels, exomes, transcriptomes and small whole‑genome projects within a compact benchtop system. Its dual‑function architecture integrates next‑generation sequencing with microarray scanning, allowing users to perform both sequencing and genotyping workflows on a single instrument, optimizing laboratory space and operational efficiency. The system supports mid‑ and high‑output flow cells, enabling flexible configuration of read length, data yield and sample throughput according to project needs. With run times ranging from approximately 12 to 30 hours depending on the chosen kit and read configuration, the NextSeq 550 delivers rapid turnaround for time‑sensitive studies. Its 2‑channel SBS technology ensures high data accuracy, while automated, load‑and‑go reagents streamline daily operation. The platform is fully compatible with Illumina’s extensive library preparation solutions and integrates seamlessly with DRAGEN‑accelerated secondary analysis through BaseSpace Sequence Hub or local servers, providing secure, scalable data processing. This combination of speed, accuracy and workflow simplicity makes the NextSeq 550 an ideal solution for laboratories seeking a reliable, scalable instrument capable of supporting evolving research demands in genomics, transcriptomics and epigenetics.

Experimental team

Instrument Scientist
  • Gabriele Scorrano
  • NAST Centre - University of Rome Tor Vergata
  • Researcher
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Staff
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  • Claudio Ottoni
  • NAST Centre - University of Rome Tor Vergata
  • Professor
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