DNA Sequencing NGS

General information


Materials Processing

Key instrumentation
Next Generation Sequencing

NextSeq 550 is a NGS system capable of: Exome Sequencing; Targeted Gene Sequencing and whole genome; total RNA-Seq, mRNA-Seq, gene expression profiling; Cytogenomic Arrays

Technical description
Description of the technique The NextSeq 550 System is an integrated platform that uses massively parallel sequencing technology for genetic analysis and functional genomics. Prepared libraries are loaded directly onto the system. Integrated cluster generation provides automated clonal amplification of single molecules randomly distributed on a glass surface. The DNA clusters thus obtained are sequenced on the NextSeq 550 using the Illumina sequencing-mediated synthesis (SBS) method with patented chemistry based on reversible terminators. In addition, the NextSeq 550 System combines microarray scanning with the sequencing system. The system offers: • Scalable from 20Gb to 120Gb on a single run to support a wide range of application and studio sizes • Runs, including clustering on the instrument, in 12-30 hours • Fully automated on-instrument cluster generation to prepare libraries and upload them directly to the instrument • The highest accuracy of any high-output benchtop sequencing system • Demonstrated SBS chemistry with single base extension makes accurate sequencing of homopolymers possible • Fully automated paired-end sequencing
Research areas and applications

Sequencing of prokaryotic and eukaryotic genomes, analysis of exomes, transcriptomes and non-coding RNA, gene expression, detection of pathogens and methylation analyses, epigenomic.

Science highlights

Experimental team

Instrument Scientist
  • Claudio Ottoni
  • NAST Centre - University of Rome Tor Vergata
  • Professor