TEM FEI

General Information

Technique

Microscopy

Key Instrumentation

Transmission Electron Microscope

The TEM FEI system at the CNR‑IPCB unit is a high‑performance transmission electron microscope designed for detailed morphological and structural characterization of inorganic, organic, polymeric, and biological materials. Operating primarily in bright‑field mode, it enables high‑resolution imaging of ultrathin specimens, allowing the investigation of nanoscale features such as lattice fringes, crystalline domains, interfaces, and internal morphologies. The instrument supports a wide range of accelerating voltages, making it suitable for both beam‑sensitive biological samples and more robust inorganic materials. Sample preparation is facilitated by an integrated cryo‑ultramicrotomy facility, which enables the production of ultrathin sections under cryogenic conditions, preserving native structures and minimizing artifacts in soft or hydrated materials. This capability is essential for studying polymers, biological tissues, vesicles, and other complex soft‑matter systems. The microscope is equipped with high‑sensitivity digital cameras that allow rapid acquisition of images and diffraction patterns, supporting both qualitative and quantitative analyses. Electron diffraction capabilities enable the identification of crystalline phases, assessment of structural order, and evaluation of orientation relationships within heterogeneous materials. The system’s stability and precise stage control allow for tilt‑series acquisition and the reconstruction of three‑dimensional structural information when required. Ancillary preparation tools, including microtomes and staining equipment, further expand the range of compatible sample types. Overall, the TEM FEI platform provides a robust and versatile solution for researchers and industrial users requiring nanoscale structural insight across diverse classes of materials, from hard inorganic systems to delicate biological assemblies.

Experimental team

Instrument Scientist
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