Confocal Microscope 3

A Leica TCS SP5 II Fluorescence Confocal Microscope is available at the University of Milano‑Bicocca, providing high‑sensitivity optical sectioning and multidimensional fluorescence imaging for advanced research in life sciences, soft matter, and functional materials. The system is built around a high‑speed resonant scanner and a conventional galvanometric scanner, enabling both rapid acquisition for live‑cell studies and high‑resolution imaging for detailed structural analysis. Its spectral detection architecture allows flexible selection of emission bands, supporting simultaneous imaging of multiple fluorophores with minimal crosstalk. The microscope is equipped with a suite of visible and near‑UV laser lines for efficient excitation of dyes, fluorescent proteins, and nanostructured probes. A tunable acousto‑optic beam splitter ensures precise control of laser power and wavelength routing, while hybrid and photomultiplier detectors provide high quantum efficiency and low noise for weak‑signal applications. The motorized XYZ stage enables automated tile scans, multi‑position experiments, and volumetric imaging with accurate spatial reproducibility. Advanced acquisition modes include lambda scanning, spectral unmixing, FRAP, FRET, and 3D reconstruction, supporting quantitative studies of molecular interactions, diffusion processes, and dynamic cellular responses. The system accommodates a broad range of objectives, from long‑working‑distance lenses for thick samples to high‑NA immersion optics for sub‑micrometric resolution. This configuration offers researchers and industrial partners a platform for high‑quality fluorescence imaging.